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KMID : 0043320130360070912
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Archives of Pharmacal Research
2013 Volume.36 No. 7 p.912 ~ p.917
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Antioxidant effect of astragalin isolated from the leaves of Morus alba L. against free radical-induced oxidative hemolysis of human red blood cells
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Choi Ji-Won
Kang Hyun-Ju
Kim Sung-Zoo
Kwon Tae-Oh
Jeong Seung-Il
Jang Seon-Il
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Abstract
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We evaluated the antioxidant properties of mulberry leaves extract (MLE) and flavonoids isolated from MLE. MLE was prepared by extraction with methanol. Flavonoids were analyzed by high-performance liquid chromatography. Oxidative hemolysis of normal human red blood cells (RBCs) was induced by the aqueous peroxyl radical [2,2′-Azobis (2-amidinopropane) dihydrochloride, AAPH]. MLE contained three flavonoids in the order quercetin (QC) > kaempferol (KF) > astragalin (AG). Oxidative hemolysis of RBCs induced by AAPH was suppressed by MLE, AG, KF, and QC in a time- and dose-dependent manner. MLE and these three flavonoids prevented the depletion of cystosolic antioxidant glutathione (GSH) in RBCs. AG had the greatest protective effect against AAPH-induced oxidative hemolysis and GSH depletion in RBCs.
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KEYWORD
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Antioxidant activity, Astragalin, Flavonoid, Mulberry leaves, Oxidative damage, Red blood cells
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